Anticorps Polyclonal de lapin anti-CCT4

• Phare
• Validé par KD/KO

CCT4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 21524-1-AP

Synonymes

CCT DELTA, CCT4, Cctd, SRB, Stimulator of TAR RNA binding, TCP 1 delta

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Galerie de données de validation

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  WB analysis of HeLa using 21524-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21524-1-AP (CCT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21524-1-AP (CCT4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 21524-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21524-1-AP (CCT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21524-1-AP (CCT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below

Informations sur le produit

21524-1-AP cible CCT4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCT4 Protéine recombinante Ag16128
• Nom complet: chaperonin containing TCP1, subunit 4 (delta)
• Masse moléculaire calculée: 539 aa, 58 kDa
• Poids moléculaire observé: 58-60 kDa
• Numéro d’acquisition GenBank: BC106934
• Symbole du gène: CCT4
• Identification du gène (NCBI): 10575
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell; CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.; Authors - Xinyu Ma ; KD Validated
human	WB	J Cell Biol; CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.; Authors - Yu-Cheng Zhang
human	WB	Mol Ther Nucleic Acids; hsa_circ_0001018 promotes papillary thyroid cancer by facilitating cell survival, invasion, G1/S cell cycle progression, and repressing cell apoptosis via crosstalk with miR-338-3p and SOX4.; Authors - Qiang Luo
human	WB	Pathog Dis; Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT.; Authors - Hirai Kimito K
human	IHC	Medicine (Baltimore); Chaperonin-containing TCP-1 subunit genes are potential prognostic biomarkers and are correlated with Th2 cell infiltration in lung adenocarcinoma: An observational study; Authors - Ruijuan Du
human	WB	Nature; In situ analysis reveals the TRiC duty cycle and PDCD5 as an open-state cofactor; Authors - Huaipeng Xing

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