Anticorps Monoclonal anti-CCT6B

CCT6B Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2F11A9

N° de cat : 67961-1-Ig

Synonymes

Chaperonin containing T-complex polypeptide 1 subunit 6B, CCT-zeta-like, CCT-zeta-2, Cctz2, CCTZ 2

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Galerie de données de validation

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  WB analysis using 67961-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67961-1-Ig (CCT6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67961-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67961-1-Ig (CCT6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67961-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67961-1-Ig (CCT6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67961-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67961-1-Ig (CCT6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 67961-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67961-1-Ig (CCT6B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 67961-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67961-1-Ig (CCT6B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 67961-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CCT6B antibody (67961-1-Ig, Clone: 2F11A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of U2OS using 67961-1-Ig

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human CCT6B (67961-1-Ig, Clone:2F11A9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HSC-T6, cellules K-562, cellules NIH/3T3
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,
• Résultats positifs en FC (Intra): cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67961-1-Ig cible CCT6B dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CCT6B Protéine recombinante Ag31295
• Nom complet: chaperonin containing TCP1, subunit 6B (zeta 2)
• Masse moléculaire calculée: 530 aa, 58 kDa
• Poids moléculaire observé: 53-58 kDa
• Numéro d’acquisition GenBank: BC027591
• Symbole du gène: CCT6B
• Identification du gène (NCBI): 10693
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCT6B, also named as CCT-zeta-2, TCP-1-zeta-like, CCT-zeta-like, Testis-specific Tcp20 and Testis-specific protein TSA303, belongs to the TCP-1 chaperonin family. CCT6B is a molecular chaperone which assists the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin. CCT6B has 3 isoforms with the molecular mass of 53-58 kDa.

Publications

Species	Application	Title
mouse	WB	Sci Rep; Molecular mechanisms of lung injury from ultra high and conventional dose rate pulsed radiation based on 4D DIA proteomics study; Authors - MengHua Li

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