Anticorps Polyclonal de lapin anti-Neprilysin/CD10

Neprilysin/CD10 Polyclonal Antibody for IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 23782-1-AP

Synonymes

MME, MME,CD10, EPN, Enkephalinase, EC:3.4.24.11

---

Galerie de données de validation

• 

  IHC staining of human kidney using 23782-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23782-1-AP (Neprilysin/CD10 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 23782-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23782-1-AP (Neprilysin/CD10 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 23782-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 23782-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human kidney using 23782-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Neprilysin/CD10 antibody (23782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 23782-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Neprilysin/CD10 antibody (23782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en IHC: tissu rénal humain, tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal humain,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

23782-1-AP cible Neprilysin/CD10 dans les applications de IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Neprilysin/CD10 Protéine recombinante Ag20634
• Nom complet: membrane metallo-endopeptidase
• Masse moléculaire calculée: 750 aa, 86 kDa
• Numéro d’acquisition GenBank: BC101632
• Symbole du gène: CD10
• Identification du gène (NCBI): 4311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).

Publications

Species	Application	Title
mouse	IF	Clin Breast Cancer; Inhibitory Impact Of Cinobufagin In Triple-Negative Breast Cancer Metastasis: Involvements Of Macrophage Reprogramming Through Upregulated MME and Inactivated FAK/STAT3 Signaling; Authors - Zhaohui Zhu

{{ptg:RelatedPrimaryAntibodies}}