Anticorps Monoclonal anti-CD107a / LAMP1

CD107a / LAMP1 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1D4B

N° de cat : CL594-65050

Synonymes

AI196048, CD107a, Lamp 1, Lamp1



Applications testées

Résultats positifs en IFcellules NIH/3T3,
Résultats positifs en cytométriecellules NIH/3T3

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-65050 cible CD107a / LAMP1 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons souris

Réactivité souris
Hôte / Isotype Rat / IgG2a, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène Fraction de la membrane plasmique de la lignée cellulaire NIH/3T3 d'embryon de souris
Nom complet lysosomal-associated membrane protein 1
Numéro d’acquisition GenBankBC006785
Symbole du gène Lamp1
Identification du gène (NCBI) 16783
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Protocole

Product Specific Protocols
IF protocol for CL594 CD107a / LAMP1 antibody CL594-65050Download protocol
FC protocol for CL594 CD107a / LAMP1 antibody CL594-65050Download protocol
Standard Protocols
Click here to view our Standard Protocols