Anticorps Monoclonal anti-CD107a / LAMP1

CD107a / LAMP1 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1D4B

N° de cat : CL750-65050

Synonymes

AI196048, CD107a, Lamp 1, Lamp1

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Galerie de données de validation

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  FC experiment of NIH/3T3 using CL750-65050

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite® Plus 750 Anti-Mouse CD107a / LAMP1 (CL750-65050, Clone:1D4B) (red), or 0.5 ug CoraLite® Plus 750 Rat IgG2a Isotype Control (2A3) (CL750-65209, Clone: 2A3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): cellules NIH/3T3
• Résultats positifs en cytométrie: cellules NIH/3T3

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-65050 cible CD107a / LAMP1 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Fraction de la membrane plasmique de la lignée cellulaire NIH/3T3 d'embryon de souris
• Nom complet: lysosomal-associated membrane protein 1
• Numéro d’acquisition GenBank: BC006785
• Symbole du gène: CD107a
• Identification du gène (NCBI): 16783
• Conjugaison: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

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