Anticorps Monoclonal anti-CD151

• Phare
• Validé par KD/KO

CD151 Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

2A8G8

N° de cat : 66567-1-Ig

Synonymes

2A8G8, CD151 antigen, GP27, Membrane glycoprotein SFA 1, Membrane glycoprotein SFA-1

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Galerie de données de validation

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  WB analysis using 66567-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66567-1-Ig (CD151 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 66567-1-Ig

  IP result of anti-CD151 (IP:66567-1-Ig, 4ug; Detection:66567-1-Ig 1:2000) with human placenta tissue lysate 960 ug.

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  IHC staining of human tonsillitis using 66567-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66567-1-Ig (CD151 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66567-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66567-1-Ig (CD151 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 66567-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD151 antibody (66567-1-Ig, Clone: 2A8G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: unboiled A431 cells, cellules A549
• Résultats positifs en IP: tissu placentaire humain,
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

66567-1-Ig cible CD151 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD151 Protéine recombinante Ag25985
• Nom complet: CD151 molecule (Raph blood group)
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC001374
• Symbole du gène: CD151
• Identification du gène (NCBI): 977
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.

Publications

Species	Application	Title
human	WB,IF	Free Radic Biol Med; Downregulation of CD151 induces oxidative stress and apoptosis in trophoblast cells via inhibiting ERK/Nrf2 signaling pathway in preeclampsia.; Authors - Zhiyin Wang ; KD Validated
human	IF	iScience; The proteomic profile of the human myotendinous junction.; Authors - Anders Karlsen
human,mouse	WB	PLoS One; Downregulation of the CD151 protects the cardiac function by the crosstalk between the endothelial cells and cardiomyocytes via exosomes; Authors - Luying Jiang
human	IF	Stem Cells Transl Med; Factor 3 regulates airway engraftment by human bronchial basal cells; Authors - Susan D Reynolds

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