Anticorps Polyclonal de lapin anti-CD19

• Phare
• Validé par KD/KO

CD19 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 27949-1-AP

Synonymes

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

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Galerie de données de validation

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  WB analysis of Raji using 27949-1-AP

  WB result of CD19 antibody (27949-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD19 transfected Raji cells.

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  WB analysis using 27949-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 27949-1-AP (CD19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27949-1-AP

  Raji cell (CD19 positive) and Jurkat (CD19 negative) were subjected to SDS PAGE followed by western blot with 27949-1-AP (CD19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat thymus using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat thymus using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Raji using 27949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD19 antibody (27949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules Raji
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de côlon de rat, tissu de côlon de souris, tissu de lymphome humain, tissu de thymus de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules Raji,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 4 publications below

Informations sur le produit

27949-1-AP cible CD19 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD19 Protéine recombinante Ag27656
• Nom complet: CD19 molecule
• Masse moléculaire calculée: 556 aa, 61 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC006338
• Symbole du gène: CD19
• Identification du gène (NCBI): 930
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.

Publications

Species	Application	Title
rat	IHC	J Adv Res; Zearalenone-14-glucoside specifically promotes dysplasia of Gut-Associated Lymphoid Tissue: A natural product for constructing intestinal nodular lymphatic hyperplasia model; Authors - Haonan Ruan
human	IHC	Int J Mol Sci; CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing; Authors - Chen Su
human	IHC	Inflamm Regen; Expression of factor XIII originating from synovial fibroblasts and macrophages induced by interleukin-6 signaling; Authors - Hirofumi Watanabe
mouse	IHC	Pharmaceuticals (Basel); Hydrogen Attenuates Inflammation by Inducing Early M2 Macrophage Polarization in Skin Wound Healing; Authors - Pengxiang Zhao