Anticorps Monoclonal anti-CD20

CD20 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2b, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

2H7

N° de cat : 65085-1-Ig

Synonymes

B lymphocyte antigen CD20, B1, Bp35, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

---

Galerie de données de validation

• 

  FC experiment of human PBMCs using 65085-1-Ig

  1X10^6 human PBMCs were surface co-stained with CL405 Anti-Human CD19 and 0.5 ug Anti-Human CD20 (65085-1-Ig, Clone: 2H7) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65085-1-Ig

  1X10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD73 (65162-1-Ig, Clone:AD2) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red) or Isotype Control. Cells were not fixed. Lymphocytes were gated.

---

Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65085-1-Ig cible CD20 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes B amygdaliennes humaines
• Nom complet: membrane-spanning 4-domains, subfamily A, member 1
• Masse moléculaire calculée: 297 aa, 33 kDa
• Numéro d’acquisition GenBank: BC002807
• Symbole du gène: CD20
• Identification du gène (NCBI): 931
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).

{{ptg:RelatedPrimaryAntibodies}}