Anticorps Monoclonal anti-CD20

CD20 Monoclonal Antibody for IF-P

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

IF-P

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4A7G3

N° de cat : CL647-60271

Synonymes

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

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Galerie de données de validation

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  IF Staining of human lung cancer using CL647-60271

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite®555 pan-keratin antibody (CL555-26411, orange).

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  IF Staining of human lung cancer using CL647-60271

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite®555 pan-keratin antibody (CL555-26411, orange).

• 

  IF Staining of human lung cancer using CL647-60271

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®488 pan-keratin antibody (CL488-26411, green).

• 

  IF Staining of human tonsillitis using CL647-60271

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL647-60271 (CD20 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en IF-P: tissu de cancer du poumon humain, tissu d'amygdalite humain

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-60271 cible CD20 dans les applications de IF-P et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD20 Protéine recombinante Ag17309
• Nom complet: membrane-spanning 4-domains, subfamily A, member 1
• Masse moléculaire calculée: 297 aa, 33 kDa
• Numéro d’acquisition GenBank: BC002807
• Symbole du gène: CD20
• Identification du gène (NCBI): 931
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

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