Anticorps Monoclonal anti-CD23

CD23 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

3H6B8

N° de cat : 60208-1-Ig

Synonymes

3H6B8, BLAST 2, BLAST-2, CD23A, CLEC4J

---

Galerie de données de validation

• 

  WB analysis using 60208-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60208-1-Ig (CD23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DC2.4 using 60208-1-Ig

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 60208-1-Ig (CD23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60208-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-1-Ig (CD23 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 60208-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD23 antibody (60208-1-Ig, Clone: 3H6B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 60208-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD23 antibody (60208-1-Ig, Clone: 3H6B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules THP-1, cellules Daudi, cellules DC2.4, cellules Raji, cellules Ramos, cellules U-937
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

60208-1-Ig cible CD23 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD23 Protéine recombinante Ag0425
• Nom complet: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Masse moléculaire calculée: 321 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC064417
• Symbole du gène: CD23
• Identification du gène (NCBI): 2208
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.

Publications

Species	Application	Title
human	IHC,IF	Clin Transl Med; Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy; Authors - Qing Wang
human	IF	Sci Signal; Short stature and combined immunodeficiency associated with mutations in RGS10.; Authors - Ivan K Chinn
mouse	WB,IF	Biol Pharm Bull; Indomethacin, a Non-steroidal Anti-inflammatory Drug, Induces Skin Dryness via PPARγ in Mice.; Authors - Kiyoko Maruyama
human	IHC	BMC Musculoskelet Disord; Anti-signal recognition particle positive necrotizing myopathy-sjogren's syndrome overlap syndrome: a descriptive study on clinical and myopathology features; Authors - Li Xu

{{ptg:RelatedPrimaryAntibodies}}