Anticorps Polyclonal de lapin anti-TEM1
TEM1 Polyclonal Antibody for WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IHC, IF, FC, CoIP, ELISA
Conjugaison
Non conjugué
N° de cat : 18160-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HUVEC, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 4 publications below |
| IF | See 5 publications below |
| FC | See 1 publications below |
| CoIP | See 1 publications below |
Informations sur le produit
18160-1-AP cible TEM1 dans les applications de WB, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | rat, Humain, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | TEM1 Protéine recombinante Ag12990 |
| Nom complet | CD248 molecule, endosialin |
| Masse moléculaire calculée | 757 aa, 81 kDa |
| Poids moléculaire observé | 160 kDa |
| Numéro d’acquisition GenBank | BC051340 |
| Symbole du gène | CD248 |
| Identification du gène (NCBI) | 57124 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
TEM1 (Tumor endothelial marker 1), also named as CD248, Endosialin and CD164L1, is a C-type lectin-like domain (CTLD) containing type I transmembrane glycoprotein. It is now considered to be a highly selective marker for activated perivascular and stromal cells, detected in most cancers and at least some inflammatory disorders. CD248 plays a role in tumor angiogenesis. It is a potential diagnostic tool and therapeutic target of inflammatory and malignant disease. Two isoforms of human TEM1 exist. The calculated molecular weights of the two isoforms are 81 kDa and 46 kDa, respectively. Native TEM1 can be glycosylated, and the glycosylated form has a larger apparent molecular weight than 81 kDa.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for TEM1 antibody 18160-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res Interaction with CD68 and regulation of GAS6 expression by endosialin in fibroblasts drives recruitment and polarization of macrophages in hepatocellular carcinoma. | ||
J Invest Dermatol Tumor Endothelial Marker 1 (TEM1/Endosialin/CD248) Enhances Wound Healing by Interacting with Platelet-Derived Growth Factor Receptors. | ||
EBioMedicine Specific loss of adipocyte CD248 improves metabolic health via reduced white adipose tissue hypoxia, fibrosis and inflammation. | ||
Oncotarget Generation of a novel Antibody-Drug Conjugate targeting endosialin: potent and durable antitumor response in sarcoma. | ||
Mol Med Antibody-drug conjugates targeting CD248 inhibits liver fibrosis through specific killing on myofibroblasts. |
