Anticorps Monoclonal anti-CD25

CD25 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG1, lambda

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

PC61.5

N° de cat : CL750-65137

Synonymes

CD25, Il2r, Il2ra, Ly 43



Applications testées

Résultats positifs en cytométrieplate-bound Anti-CD3 and Anti-CD28 stimulated mouse splenocytes,

Dilution recommandée

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-65137 cible CD25 dans les applications de FC et montre une réactivité avec des échantillons souris

Réactivité souris
Hôte / Isotype Rat / IgG1, lambda
Clonalité Monoclonal
Type Anticorps
Immunogène s/o
Nom complet interleukin 2 receptor, alpha chain
Numéro d’acquisition GenBankBC114437
Symbole du gène Il2ra
Identification du gène (NCBI) 16184
Conjugaison CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS with 0.09% sodium azide.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).

Protocole

Product Specific Protocols
FC protocol for CL Plus 750 CD25 antibody CL750-65137Download protocol
Standard Protocols
Click here to view our Standard Protocols