Anticorps Monoclonal anti-CD3e

CD3e Monoclonal Antibody for IF/ICC, FC

Hôte / Isotype

Hamster / IgG

Réactivité testée

souris

Applications

IF/ICC, FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

Publications(1)

CloneNo.

500-A2

N° de cat : CL647-65061

Synonymes

500-A2, AI504783, CD3, CD3epsilon, CD3ε

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Galerie de données de validation

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  IF Staining of mouse splenocytes using CL647-65061

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD3ε (CL647-65061, Clone: 500-A2) at dilution of 1:500.

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  FC experiment of mouse splenocytes using CL647-65061

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647-conjugated Anti-Mouse CD3ε (CL647-65061, Clone: 500-A2) (red) or 0.25 ug isotype control antibody (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en IF/ICC: splénocytes de souris,
• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65061 cible CD3e dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Hamster / IgG
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Complexes immuns isolés de cellules de thymome C6VL-BS
• Nom complet: CD3 antigen, epsilon polypeptide
• Numéro d’acquisition GenBank: BC098236
• Symbole du gène: CD3ε
• Identification du gène (NCBI): 12501
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publications

Species	Application	Title
		Cancer Cell; Overcoming tyrosine kinase inhibitor resistance in lung cancer brain metastasis with CTLA4 blockade; Authors - Minjie Fu

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