Anticorps Monoclonal anti-CD3ε

CD3ε Monoclonal Antibody for FC

Hôte / Isotype

Hamster / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

500-A2

N° de cat : 65061-1-Ig

Synonymes

AI504783, CD3, CD3e, CD3epsilon, CD3ε, T3e

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Galerie de données de validation

• 

  FC experiment of mouse splenocytes using 65061-1-Ig

  1X10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD3ε (65061-1-Ig, Clone: 500-A2) and FITC Goat anti-hamster (Armenian) IgG Antibody at dilution 1:200 (green), or stained with 0.5 ug Armenian Hamster IgG isotype Control and FITC Goat anti-hamster (Armenian) IgG Antibody at dilution 1:200 (black). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65061-1-Ig cible CD3ε dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Hamster / IgG
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Complexes immuns isolés de cellules de thymome C6VL-BS
• Nom complet: CD3 antigen, epsilon polypeptide
• Numéro d’acquisition GenBank: BC098236
• Symbole du gène: Cd3e
• Identification du gène (NCBI): 12501
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Solution saline tamponnée au borate, pH 8,2.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).