Anticorps Monoclonal anti-CD300A

CD300A Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

2F9C5

N° de cat : 67242-1-Ig

Synonymes

CD300A, CD300a molecule, CLM 8, CMRF 35 H9, CMRF 35H, CMRF35 H, CMRF35 H9, CMRF35 like molecule 8, CMRF35H, CMRF35H9, IGSF12, Inhibitory receptor protein 60, IRC1, IRC1/IRC2, IRC2, IRp60, NK inhibitory receptor

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Galerie de données de validation

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  WB analysis of THP-1 using 67242-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67242-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 67242-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 67242-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67242-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 67242-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of THP-1 using 67242-1-Ig

  1X10^6 THP-1 cells were surface stained with 0.4 ug Anti-Human CD300A (67242-1-Ig, Clone:2F9C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules HL-60, cellules Jurkat, cellules K-562, cellules Raji, tissu splénique humain
• Résultats positifs en cytométrie: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67242-1-Ig cible CD300A dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD300A Protéine recombinante Ag5776
• Nom complet: CD300a molecule
• Masse moléculaire calculée: 299 aa, 33 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC032352
• Symbole du gène: CD300A
• Identification du gène (NCBI): 11314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.