Anticorps Polyclonal de lapin anti-CD300LF

CD300LF Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 13334-1-AP

Synonymes

CD300 antigen-like family member F, CD300f, CLM 1, CLM1, CLM-1

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Galerie de données de validation

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  WB analysis using 13334-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13334-1-AP (CD300LF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 13334-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13334-1-AP (CD300LF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13334-1-AP

  IP result of anti-CD300LF (IP:13334-1-AP, 4ug; Detection:13334-1-AP 1:1500) with K-562 cells lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules U-937
• Résultats positifs en IP: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

13334-1-AP cible CD300LF dans les applications de WB, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD300LF Protéine recombinante Ag4150
• Nom complet: CD300 molecule-like family member f
• Masse moléculaire calculée: 290 aa, 32 kDa
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: BC028199
• Symbole du gène: CD300LF
• Identification du gène (NCBI): 146722
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD300LF, also known as CLM1 (CMRF35-like molecule 1), is a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. CD300LF is an inhibitory receptor that has been shown to inhibit various inflammatory processes in myeloid cells, such as macrophages and mast cells.

Publications

Species	Application	Title
	WB	BMC Pulm Med; Identification of new biomarkers for Acute Respiratory Distress Syndrome by expression-based genome-wide association study.; Authors - Dmitry N Grigoryev
human	WB	J Inflamm Res; Quercetin Attenuates MRGPRX2-Mediated Mast Cell Degranulation via the MyD88/IKK/NF-κB and PI3K/AKT/ Rac1/Cdc42 Pathway; Authors - Chenrui Zhao
human	WB,IF	Phytother Res; Quercetin Alleviates Chronic Urticaria by Negatively Regulating IgE-Mediated Mast Cell Activation Through CD300f; Authors - Chenrui Zhao ; KD Validated

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