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Anticorps Polyclonal de lapin anti-CD300LG

CD300LG Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF,ELISA

Conjugaison

Non conjugué

N° de cat : 15426-1-AP

Synonymes

CD300LG, CLM 9, CLM9, CMRF35 like molecule 9, NEPMUCIN, TREM 4, TREM4

Galerie de données de validation

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  • Western Blot (WB) analysis of human kidney tissue using CD300LG Polyclonal antibody (15426-1-AP)

    WB analysis of human kidney using 15426-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15426-1-AP (CD300LG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CD300LG Polyclonal antibody (15426-1-AP)

    WB analysis of HepG2 using 15426-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15426-1-AP (CD300LG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CD300LG Polyclonal antibody (15426-1-AP)

    IF Staining of HEK-293 using 15426-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15426-1-AP (CD300LG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Applications testées

Résultats positifs en WBtissu rénal humain, cellules HepG2
Résultats positifs en IFcellules HEK-293

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

15426-1-AP cible CD300LG dans les applications de WB, IF,ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène CD300LG Protéine recombinante Ag8075
Nom complet CD300 molecule-like family member g
Masse moléculaire calculée 36 kDa
Poids moléculaire observé 36 kDa
Numéro d’acquisition GenBankBC025395
Symbole du gène CD300LG
Identification du gène (NCBI) 146894
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD300LG, also known as CLM9 (CMRF35-like molecule 9), is a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. CD300LG is a receptor which may mediate L-selectin-dependent lymphocyte rollings and may play an important role in inflammation.

Protocole

Product Specific Protocols
WB protocol for CD300LG antibody 15426-1-APDownload protocol
IF protocol for CD300LG antibody 15426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols