Anticorps Monoclonal anti-CD31

CD31 Monoclonal Antibody for IF/ICC, FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

IF/ICC, FC

Conjugaison

Non conjugué

Publications(9)

CloneNo.

390

N° de cat : 65058-1-Ig

Synonymes

390, Pecam, PECAM 1, Pecam1, PECAM-1

---

Galerie de données de validation

• 

  IF Staining of bEnd.3 using 65058-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD31 antibody (65058-1-Ig, Clone: 390 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rat IgG(H+L).

• 

  FC experiment of mouse splenocytes using 65058-1-Ig

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD31 (65058-1-Ig, Clone: 390) and FITC anti-rat IgG2a Antibody at dilution 1:100 (red), or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-rat IgG2a Antibody at dilution 1:100 (blue). Cells were not fixed.

---

Applications testées

• Résultats positifs en IF/ICC: cellules bEnd.3,
• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 8 publications below

Informations sur le produit

65058-1-Ig cible CD31 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée de cellules progénitrices hématopoïétiques 32D de souris C3H/HeJ
• Nom complet: platelet/endothelial cell adhesion molecule 1
• Numéro d’acquisition GenBank: BC008519
• Symbole du gène: CD31
• Identification du gène (NCBI): 18613
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publications

Species	Application	Title
	IF	mBio; Human ApoE2 protects mice against Plasmodium berghei ANKA experimental cerebral malaria; Authors - Rui Liang
mouse	IF	J Ethnopharmacol; Investigation of the efficacy of Dengzhan Shengmai capsule against heart failure with preserved ejection fraction; Authors - Ziyi Kang
mouse	IF	Neuropharmacology; Δ9-tetrahydrocannabinol induces blood-brain barrier disruption: Involving the activation of CB1R and oxidative stress; Authors - Qianyao Zhang
mouse	IF	J Ethnopharmacol; Berberis dictyophylla F. inhibits angiogenesis and apoptosis of diabetic retinopathy via suppressing HIF-1α/VEGF/DLL-4/Notch-1 pathway.; Authors - Xiaopeng Ai
mouse	IF	Eur J Pharmacol; Crosstalk between endothelial cells with a non-canonical EndoMT phenotype and cardiomyocytes/fibroblasts via IGFBP5 aggravates TAC-induced cardiac dysfunction; Authors - Yue Li
		Phytother Res; A new look at angiogenesis inhibition of geniposide in experimental arthritis by blocking angiopoietin-2 exocytosis; Authors - Peirong Gan

{{ptg:RelatedPrimaryAntibodies}}