Anticorps Polyclonal de lapin anti-CD320

• Phare
• Validé par KD/KO

CD320 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 10343-1-AP

Synonymes

8D6, 8D6 antigen, 8D6A, CD320, CD320 antigen, CD320 molecule, FDC signaling molecule 8D6, FDC SM 8D6, TCblR, Transcobalamin receptor

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Galerie de données de validation

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  WB analysis using 10343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10343-1-AP (CD320 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 10343-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10343-1-AP (CD320 antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10343-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10343-1-AP (CD320 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10343-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10343-1-AP (CD320 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 10343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10343-1-AP (CD320 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10343-1-AP (CD320 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10343-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10343-1-AP (CD320 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 10343-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10343-1-AP (CD320 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 10343-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10343-1-AP (CD320 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 10343-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10343-1-AP (CD320 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HL-60 using 10343-1-AP

  1X10^6 HL-60 cells were stained with 0.2ug CD320 antibody (10343-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HEK-293, cellules Raji, tissu cardiaque de souris, tissu hépatique humain, tissu placentaire humain
• Résultats positifs en IHC: tissu rénal de souris, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HL-60

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

10343-1-AP cible CD320 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD320 Protéine recombinante Ag0344
• Nom complet: CD320 molecule
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 60-70 kDa, 58 kDa
• Numéro d’acquisition GenBank: BC000668
• Symbole du gène: CD320
• Identification du gène (NCBI): 51293
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD320, also known as 8D6, 8D6A or TCblR, is a single-pass type I membrane protein containing 2 LDL-receptor class A domains. Expressed abundantly on follicular dendritic cells (FDCs), CD320 has been shown to enhance proliferation of germinal center (GC) B cells. It is the receptor for cellular uptake of transcobalamin-bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC). CD320 has a calculated molecular mass of 29 kDa. This antibody raised against 84-280aa of human CD320 protein detects bands at 58 kDa and 60-70 kDa. The aberrant mobility of the protein by SDS-PAGE is probably caused by extensive complex glycosylation (PMID: 18779389; 23415653).

Publications

Species	Application	Title
canine, cat	IHC	Oncotarget; Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues.; Authors - Annette M Sysel
mouse	IF	Int J Biol Macromol; CD320 expression and apical membrane targeting in renal and intestinal epithelial cells.; Authors - Yue Chen
human	WB,IF	FASEB J; Identification of a novel mechanism for meso-tetra (4-carboxyphenyl) porphyrin (TCPP) uptake in cancer cells.; Authors - David J Elzi ; KD Validated
human	IHC	Anticancer Res; Immunohistochemical Quantification of the Vitamin B12 Transport Protein (TCII), Cell Surface Receptor (TCII-R) and Ki-67 in Human Tumor Xenografts.; Authors - Sysel Annette M AM
human	IHC	Heliyon; Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study; Authors - Lingyan Chen
human	IF	Cell Rep; FTY720 requires vitamin B12-TCN2-CD320 signaling in astrocytes to reduce disease in an animal model of multiple sclerosis; Authors - Deepa Jonnalagadda

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joseph (Verified Customer) (09-17-2020)

We evaluated several CD320 antibodies but the Proteintech sequence provided better specificity and greater sensitivity to the CD320 protein target.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50 dilution human; 1:50 dilution canine; 1:25 dilution feline
• Cell Tissue Type: human, canine and feline malignant various

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