Anticorps Polyclonal de lapin anti-CD40

• Phare
• Validé par KD/KO

CD40 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 28158-1-AP

Synonymes

B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5

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Galerie de données de validation

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  WB analysis using 28158-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28158-1-AP (CD40 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules Raji, cellules Ramos

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

28158-1-AP cible CD40 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD40 Protéine recombinante Ag28033
• Nom complet: CD40 molecule, TNF receptor superfamily member 5
• Masse moléculaire calculée: 277 aa, 31 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC012419
• Symbole du gène: CD40
• Identification du gène (NCBI): 958
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.

Publications

Species	Application	Title
human	WB	Toxicology; Role of miR-145-5p/ CD40 in the inflammation and apoptosis of HUVECs induced by PM2.5.; Authors - Xueting Zhang
human	WB	Cardiovasc Ther; Bazedoxifene Plays a Protective Role against Inflammatory Injury of Endothelial Cells by Targeting CD40.; Authors - Wenmin Song ; KD Validated
human	WB	FEBS Open Bio; A methylation-driven gene panel predicts survival in patients with colon cancer.; Authors - Yaojun Peng
human	IHC	Front Immunol; TANK shapes an immunosuppressive microenvironment and predicts prognosis and therapeutic response in glioma; Authors - Shasha Li