Anticorps Monoclonal anti-CD47

• Phare
• Validé par KD/KO

CD47 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (2)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(19)

CloneNo.

1E1D8

N° de cat : 66304-1-Ig

Synonymes

Leukocyte surface antigen CD47, Integrin-associated protein, Integrin associated protein, IAP, CD47 molecule

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Galerie de données de validation

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  WB analysis using 66304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66304-1-Ig

  WB result of CD47 antibody (66304-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD47 transfected A549 cells.

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  WB analysis using 66304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 66304-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CD47 antibody (66304-1-Ig, Clone: 1E1D8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Applications testées

• Résultats positifs en WB: cellules SKOV-3, cellules A549, cellules HaCaT, cellules HeLa, cellules HT-1080, cellules HUVEC, cellules Jurkat, cellules K-562, cellules MDA-MB-231, cellules NCI-H1299, cellules TF-1, tissu placentaire humain
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 17 publications below
• IF: See 3 publications below
• FC: See 1 publications below

Informations sur le produit

66304-1-Ig cible CD47 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD47 Protéine recombinante Ag14132
• Nom complet: CD47 molecule
• Masse moléculaire calculée: 323 aa, 35 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC010016
• Symbole du gène: CD47
• Identification du gène (NCBI): 961
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

Publications

Species	Application	Title
human	WB	Small; Bispecific Nanobody-Aptamer Conjugates for Enhanced Cancer Therapy in Solid Tumors; Authors - Ya Wang
human,rat	WB,IF	Redox Biol; Endothelial transferrin receptor 1 contributes to thrombogenesis through cascade ferroptosis; Authors - Haotian Ma
mouse	WB	ACS Appl Mater Interfaces; Dual-Regulated Biomimetic Nanocomposites For Promoted Tumor Photodynamic Immunotherapy; Authors - Li Liao
human,mouse	WB	J Control Release; Erythrocyte-biomimetic nanosystems to improve antitumor effects of paclitaxel on epithelial cancers.; Authors - Mingming Song
mouse	WB	J Nanobiotechnology; Bone-targeted erythrocyte-cancer hybrid membrane-camouflaged nanoparticles for enhancing photothermal and hypoxia-activated chemotherapy of bone invasion by OSCC.; Authors - Hongying Chen
human	WB	RSC Adv; Seedless preparation of Au nanorods by hydroquinone assistant and red blood cell membrane camouflage; Authors - Jing Li

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