Anticorps Polyclonal de lapin anti-CD59

• Phare
• Validé par KD/KO

CD59 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 10742-1-AP

Synonymes

16.3A5, 1F5, 1F5 antigen, CD59, CD59 glycoprotein, EJ16, EJ30, EL32, G344, HRF 20, HRF20, MAC inhibitory protein, MAC IP, MACIF, MEM43, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18 20, Protectin

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Galerie de données de validation

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  WB analysis using 10742-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10742-1-AP (CD59 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 10742-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10742-1-AP (CD59 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 10742-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 10742-1-AP (CD59 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10742-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10742-1-AP (CD59 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10742-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10742-1-AP (CD59 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 10742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10742-1-AP (CD59 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 10742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10742-1-AP (CD59 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10742-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10742-1-AP (CD59 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10742-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10742-1-AP (CD59 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10742-1-AP (CD59 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of BxPC-3 using 10742-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 10742-1-AP (CD59 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Jurkat using 10742-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CD59 (10742-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HeLa, cellules HUVEC, tissu cérébral humain
• Résultats positifs en IHC: tissu placentaire humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules BxPC-3
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10742-1-AP cible CD59 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD59 Protéine recombinante Ag1173
• Nom complet: CD59 molecule, complement regulatory protein
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC001506
• Symbole du gène: CD59
• Identification du gène (NCBI): 966
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Comparison of Fucoidans from Saccharina latissima Regarding Age-Related Macular Degeneration Relevant Pathomechanisms in Retinal Pigment Epithelium; Authors - Philipp Dörschmann
human	WB,IHC	Int J Oncol; The membrane complement regulatory protein CD59 promotes tumor growth and predicts poor prognosis in breast cancer.; Authors - Qianwen Ouyang
human	WB	PLoS One; Gecko CD59 is implicated in proximodistal identity during tail regeneration.; Authors - Wang Yongjun Y
	WB	Chem Res Toxicol; 4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.; Authors - Mikhail Linetsky
human	WB	Biochem Biophys Rep; Complement-dependent and -independent aquaporin 4-antibody-mediated cytotoxicity in human astrocytes: Pathogenetic implications in neuromyelitis optica.; Authors - S Nishiyama
human	WB,IF	Mol Cell Endocrinol; Endovascular trophoblast expresses CD59 to evade complement-dependent cytotoxicity.; Authors - Masashi Ueda ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C