Anticorps Monoclonal anti-CD68

CD68 Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG2b, kappa

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra)

Conjugaison

Non conjugué

Publications(8)

CloneNo.

Y1/82A

N° de cat : 65187-1-Ig

Synonymes

CD68 molecule, GP110, Macrosialin, SCARD1, Y1/82A

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Galerie de données de validation

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  IF Staining of THP-1 using 65187-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD68 antibody (65187-1-Ig, Clone: Y1/82A ) at dilution of 1:400 and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L), CoraLite® Plus 555 CD45 antibody (CL555-65109, Clone: HI30).

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  FC experiment of human PBMCs using 65187-1-Ig

  1X10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD68 (65187-1-Ig, Clone:Y1/82A) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

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Applications testées

• Résultats positifs en IF/ICC: cellules THP-1,
• Résultats positifs en FC (Intra): CMSP humaines
• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

65187-1-Ig cible CD68 dans les applications de WB, IHC, IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Contenu lysosomal des macrophages pulmonaires
• Nom complet: CD68 molecule
• Masse moléculaire calculée: 37 kDa
• Numéro d’acquisition GenBank: BC015557
• Symbole du gène: CD68
• Identification du gène (NCBI): 968
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Publications

Species	Application	Title
	IF	Int Immunopharmacol; Diosgenin alters LPS-induced macrophage polarization by activating PPARγ/NF-κB signaling pathway; Authors - Xingchen Xiang
	IF	Phytomedicine; Koumine regulates macrophage M1/M2 polarization via TSPO, alleviating sepsis-associated liver injury in mice; Authors - Gui-Lin Jin
		Int Immunopharmacol; Macrophages regulates the transition of pericyte to peritoneal fibrosis through the GSDMD/IL-1β axis; Authors - Qiuyuan Shao
human	IF	Hepatology; Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis; Authors - Xianzhi Liu
	IF	Exp Neurol; Microglia trigger the structural plasticity of GABAergic neurons in the hippocampal CA1 region of a lipopolysaccharide-induced neuroinflammation model; Authors - Juan Chen
	WB,IHC	Int Immunopharmacol; Inhibition of aquaporin-9 ameliorates severe acute pancreatitis and associated lung injury by NLRP3 and Nrf2/HO-1 pathways; Authors - Jiawei Chen

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