Anticorps Monoclonal anti-CD68

CD68 Monoclonal Antibody for IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(86)

CloneNo.

3A9A7

Avis (2)

N° de cat : 66231-2-Ig

Synonymes

3A9A7, CD68 molecule, GP110, Macrosialin, SCARD1

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Galerie de données de validation

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  IHC staining of human tonsillitis using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 66231-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD68 antibody (66231-2-Ig, Clone: 3A9A7 ) at dilution of 1:6000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 66231-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD68 antibody (66231-2-Ig, Clone: 3A9A7 ) at dilution of 1:6000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu d'amygdalite humain, tissu d'appendicite humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de cancer du poumon humain, tissu de carcinome urothélial humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:1000-1:6000
• Immunofluorescence (IF)-P: IF-P : 1:3000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 32 publications below
• IF: See 51 publications below

Informations sur le produit

66231-2-Ig cible CD68 dans les applications de IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD68 Protéine recombinante Ag22815
• Nom complet: CD68 molecule
• Masse moléculaire calculée: 37 kDa
• Numéro d’acquisition GenBank: BC015557
• Symbole du gène: CD68
• Identification du gène (NCBI): 968
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Publications

Species	Application	Title
human	IF	Cell Rep Med; Microneedle delivery of CAR-M-like engineered macrophages alleviates intervertebral disc degeneration through enhanced efferocytosis capacity; Authors - Xingyu Zhou
	IHC	J Clin Invest; Lactate inhibits ATP6V0d2 expression in tumor-associated macrophages to promote HIF-2α-mediated tumor progression.; Authors - Na Liu
	IF	Adv Healthc Mater; Wireless Electric Cues Mediate Autologous DPSC-Loaded Conductive Hydrogel Microspheres to Engineer The Immuno-Angiogenic Niche for Homologous Maxillofacial Bone Regeneration; Authors - Jiwei Sun
		Redox Biol; Quercetin hinders microglial activation to alleviate neurotoxicity via the interplay between NLRP3 inflammasome and mitophagy.; Authors - Xiaojuan Han
	IHC	Redox Biol; Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury.; Authors - Ming Kong
human	IF	Transl Res; Purinergic receptor P2 × 7 contributes to abdominal aortic aneurysm development via modulating macrophage pyroptosis and inflammation; Authors - Likun Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cohen (Verified Customer) (06-02-2025)

Received a generous test sample of 20ul which stretched far. While the suggested dilutions ranged from 1/3000 to 12000, I found that 1/3000 was optimal for human adipose tissue and ectopic endometrial tissue. This is very impressive for a primary antibody, in my experience.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/3000
• Cell Tissue Type: Human adipose and ectopic endometrial tissue

FH

Balawant (Verified Customer) (12-25-2023)

This excellent antibody has no background.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: colon tissue

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