Anticorps Recombinant de lapin anti-CD79a

CD79a Recombinant Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

HM57

N° de cat : 65610-1-MR

Synonymes

Membrane-bound immunoglobulin-associated protein, MB-1 membrane glycoprotein, MB1, MB 1 membrane glycoprotein, MB 1

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Galerie de données de validation

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  FC experiment of human PBMCs using 65610-1-MR

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD79a (HM57) Mouse IgG2a RecAb (65610-1-MR, Clone:HM57) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG2a. Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65610-1-MR

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD79a (HM57) Mouse IgG2a RecAb (65610-1-MR, Clone:HM57)(red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4)(blue) and PE-conjugated Goat Anti-Mouse IgG2a. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated.

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Applications testées

• Résultats positifs en FC (Intra): CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65610-1-MR cible CD79a dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Synthetic peptide corresponding to 202-216 amino acid sequence of human CD79a
• Nom complet: CD79a molecule, immunoglobulin-associated alpha
• Numéro d’acquisition GenBank: BC113731
• Symbole du gène: CD79A
• Identification du gène (NCBI): 973
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79a is expressed in B cell and localizes in the cell membrane. CD79a is required in cooperation with CD79b for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79a is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

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