Anticorps Recombinant de lapin anti-CD79a

CD79a Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

241299F3

N° de cat : 98072-1-RR

Synonymes

241299F3, B-cell antigen receptor complex-associated protein alpha chain, MB 1, MB 1 membrane glycoprotein, MB1

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Galerie de données de validation

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  FC experiment of human PBMCs using 98072-1-RR

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD79a Rabbit RecAb (98072-1-RR, Clone: 241299F3) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98072-1-RR

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD79a Rabbit RecAb (98072-1-RR, Clone: 241299F3) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated.

• 

  Affinity and Kinetic Characterization of 98072-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98072-1-RR against Human CD79a were performed. The affinity constant is 0.151 nM.

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Applications testées

• Résultats positifs en FC (Intra): CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98072-1-RR cible CD79a dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD79a molecule, immunoglobulin-associated alpha
• Masse moléculaire calculée: 226 aa, 25 kDa
• Numéro d’acquisition GenBank: BC113733
• Symbole du gène: CD79A
• Identification du gène (NCBI): 973
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cells and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

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