Anticorps Recombinant de lapin anti-CD79a

CD79a Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241231F7

N° de cat : CL647-98129

Synonymes

241231F7

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Galerie de données de validation

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  FC experiment of mouse splenocytes using CL647-98129

  1x10^6 mouse splenocytes were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD79a Rabbit RecAb (CL647-98129, Clone: 241231F7) or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were co-stained with CoraLite® Plus 488 Anti-Mouse CD45R (B220) (RA3-6B2) (CL488-65139, Clone: RA3-6B2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of mouse splenocytes using CL647-98129

  1x10^6 mouse splenocytes were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD79a Rabbit RecAb (CL647-98129, Clone: 241231F7) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-98129 cible CD79a dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD79a Protéine recombinante Eg1388
• Nom complet: CD79A antigen (immunoglobulin-associated alpha)
• Masse moléculaire calculée: 25 kDa
• Numéro d’acquisition GenBank: NM_007655.3
• Symbole du gène: Cd79a
• Identification du gène (NCBI): 12518
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cell and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

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