Anticorps Monoclonal anti-CD86

CD86 Monoclonal Antibody for IF/ICC, FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

IF/ICC, FC

Conjugaison

Non conjugué

Publications(2)

CloneNo.

BU63

Avis (1)

N° de cat : 65165-1-Ig

Synonymes

Activation B7 2 antigen, B7 2, B70, BU63, CD28LG2, CD86, CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72

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Galerie de données de validation

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  IF Staining of Raji using 65165-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD86 antibody (65165-1-Ig, Clone: BU63 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  FC experiment of Daudi using 65165-1-Ig

  1X10^6 Daudi cells were surface stained with 0.20 ug Anti-Human CD86 (65165-1-Ig, Clone: BU63) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were not fixed.

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Applications testées

• Résultats positifs en IF/ICC: cellules Raji,
• Résultats positifs en cytométrie: cellules Daudi,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

65165-1-Ig cible CD86 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée cellulaire B-lymphoblastoïde ARH 77
• Nom complet: CD86 molecule
• Masse moléculaire calculée: 329 aa, 38 kDa
• Numéro d’acquisition GenBank: BC040261
• Symbole du gène: CD86
• Identification du gène (NCBI): 942
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Publications

Species	Application	Title
human	IF	J Agric Food Chem; Novel Collagen Analogs with Multicopy Mucin-Type Sequences for Multifunctional Enhancement Properties Using SUMO Fusion Tags; Authors - Zilong Zhao
human	FC	Int Immunopharmacol; Isosilybin A exhibits anti-inflammatory properties in rosacea by inhibiting MAPK pathway and M1 macrophage polarization; Authors - Chen-Chen Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paul (Verified Customer) (02-13-2025)

Works well for immunofluorescence. Clear signal in high expressing B cell line at 1/400 dilution.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/400
• Cell Tissue Type: B cell lymphoma

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