Anticorps Monoclonal anti-CD86
CD86 Monoclonal Antibody for IF/ICC, FC
Hôte / Isotype
Mouse / IgG1, kappa
Réactivité testée
Humain
Applications
IF/ICC, FC
Conjugaison
Non conjugué
CloneNo.
BU63
N° de cat : 65165-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en IF/ICC | cellules Raji, |
| Résultats positifs en cytométrie | cellules Daudi, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| IF | See 1 publications below |
| FC | See 1 publications below |
Informations sur le produit
65165-1-Ig cible CD86 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | Humain |
| Hôte / Isotype | Mouse / IgG1, kappa |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | Lignée cellulaire B-lymphoblastoïde ARH 77 |
| Nom complet | CD86 molecule |
| Masse moléculaire calculée | 329 aa, 38 kDa |
| Numéro d’acquisition GenBank | BC040261 |
| Symbole du gène | CD86 |
| Identification du gène (NCBI) | 942 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine G |
| Tampon de stockage | PBS with 0.09% sodium azide |
| Conditions de stockage | Store at 2-8°C. Stable for one year after shipment. |
Informations générales
CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.
Protocole
| Product Specific Protocols | |
|---|---|
| IF protocol for CD86 antibody 65165-1-Ig | Download protocol |
| FC protocol for CD86 antibody 65165-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Agric Food Chem Novel Collagen Analogs with Multicopy Mucin-Type Sequences for Multifunctional Enhancement Properties Using SUMO Fusion Tags | ||
Int Immunopharmacol Isosilybin A exhibits anti-inflammatory properties in rosacea by inhibiting MAPK pathway and M1 macrophage polarization |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Paul (Verified Customer) (02-13-2025) | Works well for immunofluorescence. Clear signal in high expressing B cell line at 1/400 dilution.
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