Anticorps Recombinant de lapin anti-CD8b

CD8b Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

241326A7

N° de cat : CL594-98103

Synonymes

CD8, 241326A7, CD8b molecule, CD8B1, Leu2

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Galerie de données de validation

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  FC experiment of human PBMCs using CL594-98103

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 5 ul CoraLite®594 Anti-Human CD8b Rabbit RecAb (CL594-98103, Clone:241326A7), or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9).Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of human PBMCs using CL594-98103

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD8b Rabbit RecAb (CL594-98103, Clone:241326A7)(red), or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9)(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-98103 cible CD8b dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD8b molecule
• Masse moléculaire calculée: 243 aa, 27 kDa
• Numéro d’acquisition GenBank: BC100913
• Symbole du gène: CD8B
• Identification du gène (NCBI): 926
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD8b (T-cell surface glycoprotein CD8 beta chain) is an integral membrane glycoprotein that forms disulfide-linked heterodimers with CD8a (CD8 alpha chain). The CD8 alpha/beta heterodimer is the predominant CD8 complex expressed on the cell surface (PMID: 1534146; 2111591). CD8 is a transmembrane glycoprotein predominantly expressed on the surface of cytotoxic T cells and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells.

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