Anticorps Monoclonal anti-CD97

CD97 Monoclonal Antibody for FC, IF, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IF, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

3F10C2

N° de cat : 66972-1-Ig

Synonymes

CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, TM7LN1

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Galerie de données de validation

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  WB analysis of K-562 using 66972-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 66972-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of DC2.4 using 66972-1-Ig

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66972-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 66972-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD97 antibody (66972-1-Ig, Clone: 3F10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of Jurkat using 66972-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human CD97 (66972-1-Ig, Clone:3F10C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules DC2.4, cellules HepG2, cellules HL-60
• Résultats positifs en IF: cellules Jurkat,
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66972-1-Ig cible CD97 dans les applications de WB, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD97 Protéine recombinante Ag27991
• Nom complet: CD97 molecule
• Masse moléculaire calculée: 722 aa, 79 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC026690
• Symbole du gène: CD97
• Identification du gène (NCBI): 976
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C