Anticorps Polyclonal de lapin anti-Cdc20

• Phare
• Validé par KD/KO

Cdc20 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

31

N° de cat : 10252-1-AP

Synonymes

CDC20, CDC20A, p55CDC

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Galerie de données de validation

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  WB analysis using 10252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10252-1-AP (Cdc20 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10252-1-AP (Cdc20 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10252-1-AP

  WB result of Cdc20 antibody (10252-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cdc20 transfected HeLa cells.

• 

  WB analysis using 10252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10252-1-AP (Cdc20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10252-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10252-1-AP (CDC20 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10252-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10252-1-AP (CDC20 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 10252-1-AP

  IP Result of anti-CDC20 (IP:10252-1-AP, 4ug; Detection:10252-1-AP 1:800) with Jurkat cells lysate 3200ug.

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  IHC staining of human breast cancer using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10252-1-AP (Cdc20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10252-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cdc20 antibody (10252-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules HL-60, cellules Jurkat, cellules PC-3
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu de carcinome urothélial humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:14000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 25 publications below
• IHC: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

10252-1-AP cible Cdc20 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cdc20 Protéine recombinante Ag0295
• Nom complet: cell division cycle 20 homolog (S. cerevisiae)
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC001088
• Symbole du gène: CDC20
• Identification du gène (NCBI): 991
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).

Publications

Species	Application	Title
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human	WB	Elife; Ordered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exit.; Authors - James Holder
human	WB	Int J Mol Sci; Downregulation of CDC20 Increases Radiosensitivity through Mcl-1/p-Chk1-Mediated DNA Damage and Apoptosis in Tumor Cells.; Authors - Yang Gao ; KD Validated
human	WB	Cell Death Dis; Upregulation of kinesin family member 4A enhanced cell proliferation via activation of Akt signaling and predicted a poor prognosis in hepatocellular carcinoma.; Authors - Yanlin Huang
human	WB	Aging (Albany NY); The bioinformatics and experimental analysis of the novel roles of virus infection-associated gene CDC20 for prognosis and immune infiltration in hepatocellular carcinoma.; Authors - Juanni Li

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