Anticorps Polyclonal de lapin anti-RASGRF1

• Phare
• Validé par KD/KO

RASGRF1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (1)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 12958-1-AP

Synonymes

CDC25, CDC25L, GNRP, GRF1, GRF55

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Galerie de données de validation

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  WB analysis of rat brain using 12958-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12958-1-AP (RASGRF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 12958-1-AP

  IP result of anti-RASGRF1 (IP:12958-1-AP, 4ug; Detection:12958-1-AP 1:600) with rat brain tissue lysate 1120 ug.

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  IHC staining of rat brain using 12958-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12958-1-AP (RASGRF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12958-1-AP (RASGRF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat,
• Résultats positifs en IP: tissu cérébral de rat,
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Informations sur le produit

12958-1-AP cible RASGRF1 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RASGRF1 Protéine recombinante Ag4072
• Nom complet: Ras protein-specific guanine nucleotide-releasing factor 1
• Masse moléculaire calculée: 134 kDa
• Poids moléculaire observé: 130-140 kDa
• Numéro d’acquisition GenBank: BC040275
• Symbole du gène: RASGRF1
• Identification du gène (NCBI): 5923
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RasGRF1, a member of calmodulin-activated guanine-nucleotide exchange factors (GEFs), highly expressed at the synaptic junctions of the central nervous system (CNS). It is similar to the Saccharomyces cerevisiae CDC25. RASGRF1 serves as an in vivo activator for H-RAS and members of the R-RAS and RAC subfamilies. Several studies have shown that RasGRF1 also binds to microtubules and participate in the regulation of neurite outgrowth. RasGRF1 has been implicated in neuronal excitability. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.

Publications

Species	Application	Title
rat	WB,IHC	Aging (Albany NY); Upregulation of RasGRF1 ameliorates spatial cognitive dysfunction in mice after chronic cerebral hypoperfusion; Authors - Li-Jie Yang ; KD Validated
mouse	WB	J Agric Food Chem; Anthocyanins from Black Chokeberry (Aroniamelanocarpa Elliot) Delayed Aging-Related Degenerative Changes of Brain.; Authors - Jie Wei
		Leuk Lymphoma; Amplification of B cell receptor-Erk signaling by Rasgrf-1 over-expression in chronic lymphocytic leukemia.; Authors - Wei Liao
mouse	WB	Biomaterials; LDH nanoparticles-doped cellulose nanofiber scaffolds with aligned microchannels direct high-efficiency neural regeneration and organized neural circuit remodeling through RhoA/Rock/Myosin II pathway; Authors - Xuening Pang
human,mouse	WB,IHC	Bone Res; IGF2BP3-mediated m6A modification of RASGRF1 promoting joint injury in rheumatoid arthritis; Authors - Qishun Geng ; KD Validated

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