Anticorps Monoclonal anti-CDC25C

CDC25C Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

2B6B12

Avis (1)

N° de cat : 66912-1-Ig

Synonymes

CDC25, CDC25C, M phase inducer phosphatase 3

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Galerie de données de validation

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  WB analysis using 66912-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 66912-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 66912-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.2 ug Anti-Human CDC25C (66912-1-Ig, Clone:2B6B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules PC-12, cellules Raji
• Résultats positifs en cytométrie: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

66912-1-Ig cible CDC25C dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDC25C Protéine recombinante Ag10240
• Nom complet: cell division cycle 25 homolog C (S. pombe)
• Masse moléculaire calculée: 473 aa, 53 kDa
• Poids moléculaire observé: 46-53 kDa
• Numéro d’acquisition GenBank: BC019089
• Symbole du gène: CDC25C
• Identification du gène (NCBI): 995
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C (cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells (PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop (PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa (PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.

Publications

Species	Application	Title
mouse	WB	Reprod Toxicol; High-dose zearalenone exposure disturbs G2/M transition during mouse oocyte maturation.; Authors - Yi-Ming Ji
mouse	WB	Oncol Lett; Luteolin potentiates low-dose oxaliplatin-induced inhibitory effects on cell proliferation in gastric cancer by inducing G2/M cell cycle arrest and apoptosis.; Authors - Jun Ma
human	WB	Food Chem Toxicol; DON entry into the nucleus induces DNA damage, apoptosis and cycle arrest in GES-1 cells; Authors - Silu Hou
mouse	WB	Toxicology; ATM deficiency aggravates the progression of liver fibrosis induced by carbon tetrachloride in mice; Authors - Ming Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Angélique (Verified Customer) (11-25-2021)

on 20ug total proteins

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: murine hepatocytes

CDC25C Antibody Western Blot validation (1/2000 dilution) in murine hepatocytes (Cat no:66912-1-Ig)