Anticorps Monoclonal anti-CDC42

CDC42 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2G5E8

N° de cat : 67212-1-Ig

Synonymes

2G5E8, EC:3.6.5.2, G25K, G25K GTP binding protein, G25K GTP-binding protein

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Galerie de données de validation

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  WB analysis using 67212-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67212-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 67212-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67212-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67212-1-Ig cible CDC42 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDC42 Protéine recombinante Ag28550
• Nom complet: cell division cycle 42 (GTP binding protein, 25kDa)
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC002711
• Symbole du gène: CDC42
• Identification du gène (NCBI): 998
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cdc42, the human homologue of the cell division cycle 42 of Saccharomyces cerevisiae, belongs to the Rho/Rac subfamily of Ras-like GTPases. It acts as molecular switches to regulate numerous cellular responses including the organization of the actin cytoskeleton, gene transcription, cell cycle progression, and membrane trafficking

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