Anticorps Polyclonal de lapin anti-CDC45L

• Phare
• Validé par KD/KO

CDC45L Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 15678-1-AP

Synonymes

CDC45, CDC45L, CDC45L2, PORC PI 1

---

Galerie de données de validation

• 

  WB analysis of K-562 using 15678-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15678-1-AP (CDC45L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 15678-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15678-1-AP (CDC45L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15678-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15678-1-AP (CDC45L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15678-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15678-1-AP (CDC45L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15678-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15678-1-AP (CDC45L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 15678-1-AP

  IP Result of anti-CDC45L (IP:15678-1-AP, 3ug; Detection:15678-1-AP 1:1000) with K-562 cells lysate 3400ug.

• 

  IHC staining of mouse testis using 15678-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15678-1-AP (CDC45L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15678-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15678-1-AP (CDC45L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15678-1-AP

  Immunofluorescent analysis of Hela cells, using CDC45L antibody 15678-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 15678-1-AP

  1X10^6 HeLa cells were stained with 0.2ug CDC45L antibody (15678-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293, cellules HeLa, cellules Jurkat, tissu de thymus de souris
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

15678-1-AP cible CDC45L dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDC45L Protéine recombinante Ag8150
• Nom complet: CDC45 cell division cycle 45-like (S. cerevisiae)
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 60-66 kDa
• Numéro d’acquisition GenBank: BC010022
• Symbole du gène: CDC45L
• Identification du gène (NCBI): 8318
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDC45L(Cell division control protein 45 homolog), plays a role in the initiation of DNA replication, and genetically interacts with MCM family members and with ORC2 and physically form a complex including Mcm5p. It's the first gene missed in DiGeorge syndrome that suggest involve in cell proliferation. This is a rabbit polyclonal antibody raised against part of C-terminus of human CDC45L.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; The CRL4DTL E3 ligase induces degradation of the DNA replication initiation factor TICRR/TRESLIN specifically during S phase.; Authors - Kimberlie A Wittig ; KD Validated
human	WB	Oncotarget; The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.; Authors - Lifei Fan
human	IHC	Ann Transl Med; Nine hub genes related to the prognosis of HBV-positive hepatocellular carcinoma identified by protein interaction analysis.; Authors - Wenhui Xie
human	WB	Signal Transduct Target Ther; LSD1 is required for euchromatic origin firing and replication timing.; Authors - Yue Wang
human	WB	Cell Cycle; IGF2BP2 promotes glycolysis and hepatocellular carcinoma stemness by stabilizing CDC45 mRNA via m6A modification; Authors - Tao Wu ; KD Validated