Anticorps Polyclonal de lapin anti-CDC6

• Phare
• Validé par KD/KO

CDC6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 11640-1-AP

Synonymes

Cdc18 related protein, CDC18L, CDC6, CDC6 related protein, HsCDC18, HsCDC6, p62(cdc6)

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Galerie de données de validation

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  WB analysis of Jurkat using 11640-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11640-1-AP (CDC6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11640-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11640-1-AP (CDC6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11640-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11640-1-AP (CDC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 11640-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 11640-1-AP (CDC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11640-1-AP

  IP result of anti-CDC6(IP:11640-1-AP, 4ug; Detection:11640-1-AP 1:1000) with HeLa cells lysate 1360 ug.

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  IHC staining of mouse brain using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11640-1-AP (CDC6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11640-1-AP (CDC6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11640-1-AP (CDC6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11640-1-AP (CDC6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11640-1-AP (CDC6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11640-1-AP (CDC6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11640-1-AP

  Immunofluorescent analysis of HepG2 cells, using CDC6 antibody 11640-1-AP at 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules SMMC-7721
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du côlon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

11640-1-AP cible CDC6 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDC6 Protéine recombinante Ag2228
• Nom complet: cell division cycle 6 homolog (S. cerevisiae)
• Masse moléculaire calculée: 560 aa, 63 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC025232
• Symbole du gène: CDC6
• Identification du gène (NCBI): 990
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. The active Cdc6 functioning as a dimer at replication origins (10793143,15096526). It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CDC6.

Publications

Species	Application	Title
human	WB	Mol Cancer; A novel LncRNA transcript, RBAT1, accelerates tumorigenesis through interacting with HNRNPL and cis-activating E2F3.; Authors - Xiaoyu He
human	IF	Proc Natl Acad Sci U S A; Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase.; Authors - Yim Hyungshin H
mouse	IF	PLoS Genet; Histone deacetylase 2 (HDAC2) regulates chromosome segregation and kinetochore function via H4K16 deacetylation during oocyte maturation in mouse.; Authors - Ma Pengpeng P
human	WB	Int J Mol Sci; Norcantharidin Induces Immunogenic Cell Death of Bladder Cancer Cells through Promoting Autophagy in Acidic Culture.; Authors - Lili Xu
human	WB	Aging (Albany NY); β-Elemene enhances radiosensitivity in non-small-cell lung cancer by inhibiting epithelial-mesenchymal transition and cancer stem cell traits via Prx-1/NF-kB/iNOS signaling pathway.; Authors - Kun Zou
human	WB,IHC	Eur J Cancer; Y-box binding protein-1 (YB-1) promotes cell cycle progression through CDC6-dependent pathway in human cancer cells.; Authors - Basaki Yuji Y ; KD Validated