Anticorps Polyclonal de lapin anti-CDCA8

• Phare
• Validé par KD/KO

CDCA8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 12465-1-AP

Synonymes

BOR, Borealin, Cell division cycle-associated protein 8, Dasra B, Dasra-B

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Galerie de données de validation

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  WB analysis of mouse kidney using 12465-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12465-1-AP (CDCA8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12465-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12465-1-AP (CDCA8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 12465-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12465-1-AP (CDCA8 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 12465-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12465-1-AP (CDCA8 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 12465-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CDCA8 antibody (12465-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HeLa using 12465-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12465-1-AP (CDCA8 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

12465-1-AP cible CDCA8 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDCA8 Protéine recombinante Ag3136
• Nom complet: cell division cycle associated 8
• Masse moléculaire calculée: 280 aa, 31 kDa
• Poids moléculaire observé: 31-38 kDa
• Numéro d’acquisition GenBank: BC016944
• Symbole du gène: CDCA8
• Identification du gène (NCBI): 55143
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDCA8, also named as Borealin, Dasra-B and PESCRG3, is a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. This protein can be migrated to 35-40 kDa with phosphor modification or ~50 kDa with SUMO modification.

Publications

Species	Application	Title
human	IF	Science; ESCRT-III governs the Aurora B-mediated abscission checkpoint through CHMP4C.; Authors - Jeremy G Carlton
human	IHC	Front Pharmacol; A novel risk model of three SUMOylation genes based on RNA expression for potential prognosis and treatment sensitivity prediction in kidney cancer; Authors - Song-Chao Li
human	WB,IHC	J Cell Mol Med; Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma; Authors - Xiao-Han Cui
mouse	WB	Cell Mol Life Sci; Global phosphoproteomic analysis identified key kinases regulating male meiosis in mouse; Authors - Haojie Li
human	WB,IHC	Funct Integr Genomics; Let-7c-5p down-regulates immune-related CDCA8 to inhibit hepatocellular carcinoma; Authors - Wanjin Chen
human	WB,IHC	Am J Transl Res; Cell division cycle associated 8 promotes the growth and inhibits the apoptosis of endometrial cancer cells by regulating cell cycle and P53/Rb signaling pathway; Authors - Wenxue Li ; KD Validated

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