Anticorps Polyclonal de lapin anti-E-cadherin

E-cadherin Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 20648-1-AP

Synonymes

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Galerie de données de validation

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  WB analysis of PC-3 using 20648-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20648-1-AP (E-cadherin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 20648-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 20648-1-AP (E-cadherin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 20648-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20648-1-AP (E-cadherin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 20648-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20648-1-AP (E-cadherin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 20648-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20648-1-AP (E-cadherin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 20648-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20648-1-AP (E-cadherin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 20648-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 20648-1-AP (E-cadherin antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 20648-1-AP

  Immunofluorescent analysis of HepG2 cells, using CDH1 antibody 20648-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 20648-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug E-cadherin antibody (20648-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:200.

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Applications testées

• Résultats positifs en WB: cellules PC-3
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

20648-1-AP cible E-cadherin dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cadherin 1, type 1, E-cadherin (epithelial)
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 100-125 kDa
• Numéro d’acquisition GenBank: NM_004360
• Symbole du gène: CDH1
• Identification du gène (NCBI): 999
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. This antibody is specific to CDH1.

Publications

Species	Application	Title
human	WB	Cancer Lett; SDF-1/CXCR4 Promotes epithelial-mesenchymal transition and progression of colorectal cancer by activation of the wnt/β-catenin signaling pathway.; Authors - Ting-Hua Hu
human	WB	Onco Targets Ther; Bioinformatics Analyses Indicate That Cathepsin G (CTSG) is a Potential Immune-Related Biomarker in Oral Squamous Cell Carcinoma (OSCC).; Authors - Guang-Zhao Huang
human	WB, IF	Endocrine; Adipogenic changes of hepatocytes in a high-fat diet-induced fatty liver mice model and non-alcoholic fatty liver disease patients.; Authors - Xiaoli Pan
mouse	IF	Tissue Cell; Slc44a2 deletion alters tetraspanin and N-cadherin expression: Reduced adhesion and enhanced proliferation in cultured mesenchymal lung cells.; Authors - Thankam S Nair
human	WB	Mol Med Rep; MicroRNA-590 is an EMT-suppressive microRNA involved in the TGFβ signaling pathway.; Authors - Tianming Liu
human	WB	Int J Mol Sci; Androgen-Responsive Oncogenic lncRNA RP11-1023L17.1 Enhances c-Myc Protein Stability in Prostate Cancer; Authors - Wenhua Huang

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