Anticorps Polyclonal de lapin anti-Cadherin-16

• Phare
• Validé par KD/KO

Cadherin-16 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 15107-1-AP

Synonymes

cadherin 16, KSP cadherin, Cadherin-16, CDH16, Kidney specific cadherin, Ksp cadherin

---

Galerie de données de validation

• 

  WB analysis using 15107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15107-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15107-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 15107-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15107-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 15107-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 15107-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 15107-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HEK-293 using 15107-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug Cadherin-16 antibody (15107-1-AP, red) and control antibody (blue). Fixed with blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293, tissu rénal de rat, tissu rénal humain
• Résultats positifs en IHC: tissu rénal humain, tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 4 publications below

Informations sur le produit

15107-1-AP cible Cadherin-16 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cadherin-16 Protéine recombinante Ag4343
• Nom complet: cadherin 16, KSP-cadherin
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC027912
• Symbole du gène: CDH16
• Identification du gène (NCBI): 1014
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherin-16 (CDH16), also known as kidney-specific cadherin (Ksp-cadherin), is a tissue-specific member of the cadherin family of adhesion molecules (PMID: 7615566). It is a glycoprotein expressed by tubular epithelial cells in the kidney. Cadherin-16 expression is down-regulated in renal cell carcinoma, suggesting a role in tumor suppression (PMID: 15886705).

Publications

Species	Application	Title
mouse	IF	Int Immunopharmacol; Cytoplasmic HMGB1 induces renal tubular ferroptosis after ischemia/reperfusion; Authors - Zhi Zhao
mouse	WB,IF	Front Cell Dev Biol; Transient Receptor Potential Channel 6 Knockout Ameliorates Kidney Fibrosis by Inhibition of Epithelial-Mesenchymal Transition.; Authors - Yanhong Zhang
mouse	WB,IF	J Cell Sci; Pax8 controls thyroid follicular polarity through Cadherin-16.; Authors - Petrina Koumarianou ; KD Validated
human	WB	J Cancer; TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers.; Authors - Jiaqi Dai
human	WB	Oncol Lett; Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.; Authors - Xiaolin Yang
mouse	IF	Cell Death Dis; Blocking connexin 43 and its promotion of ATP release from renal tubular epithelial cells ameliorates renal fibrosis.; Authors - Huzi Xu

Produits associés

E-cadherin Polyclonal antibody

20874-1-AP

PAX8 Polyclonal antibody

10336-1-AP

MME,CD10 Polyclonal antibody

18008-1-AP

Vimentin Polyclonal antibody

10366-1-AP