Anticorps Polyclonal de lapin anti-CDK1

• Phare
• Validé par KD/KO

CDK1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

55

N° de cat : 10762-1-AP

Synonymes

CDC2, CDC28A, CDK1, Cell division protein kinase 1, Cyclin dependent kinase 1, p34 protein kinase

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Galerie de données de validation

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  WB analysis using 10762-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10762-1-AP (CDK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10762-1-AP

  WB result of CDK1 antibody (10762-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK1 transfected HeLa cells.

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  WB analysis of HeLa using 10762-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10762-1-AP (CDK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10762-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10762-1-AP (CDK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10762-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10762-1-AP (CDK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10762-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10762-1-AP (CDK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10762-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10762-1-AP (CDK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10762-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK1 antibody (10762-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HeLa using 10762-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK1 (10762-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellule C2C12, cellules C2C12, cellules HeLa, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 52 publications below
• IHC: See 3 publications below

Informations sur le produit

10762-1-AP cible CDK1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDK1 Protéine recombinante Ag1183
• Nom complet: cell division cycle 2, G1 to S and G2 to M
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC014563
• Symbole du gène: CDC2
• Identification du gène (NCBI): 983
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa. This antibody may have cross reaction with CDK2.

Publications

Species	Application	Title
human	WB	Elife; PMID: 30132758; Authors - Tonya Kueck
human	WB	Biomed Pharmacother; Osthole inhibits the PI3K/AKT signaling pathway via activation of PTEN and induces cell cycle arrest and apoptosis in esophageal squamous cell carcinoma.; Authors - Xinbing Zhu
mouse	WB	Mol Ther Nucleic Acids; Inhibition of the lncRNA MIAT prevents podocyte injury and mitotic catastrophe in diabetic nephropathy.; Authors - Ziyang Wang
human	WB	Cell Death Dis; PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT.; Authors - Wen-Kai Shi
human	WB	Cancers (Basel); Extracellular Vesicle Delivery of TRAIL Eradicates Resistant Tumor Growth in Combination with CDK Inhibition by Dinaciclib.; Authors - Changhong Ke
human	WB	Life Sci; Periplocin inhibits hepatocellular carcinoma progression and reduces the recruitment of MDSCs through AKT/NF-κB pathway; Authors - Jia-Peng Lin