Anticorps Polyclonal de lapin anti-CDK3

CDK3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 55103-1-AP

Synonymes

CDKN3, Cell division protein kinase 3, Cyclin dependent kinase 3, Cyclin-dependent kinase 3, EC:2.7.11.22

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Galerie de données de validation

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  WB analysis using 55103-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55103-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 55103-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 55103-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 55103-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55103-1-AP (CDK3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55103-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55103-1-AP (CDK3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 55103-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK3 antibody (55103-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 55103-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK3 Polyclonal antibody (55103-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules Jurkat, cellules MCF-7, tissu cérébral de rat
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

55103-1-AP cible CDK3 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cyclin-dependent kinase 3
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: NM_001258
• Symbole du gène: CDK3
• Identification du gène (NCBI): 1018
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDK3 belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It is probably involved in the control of the cell cycle. CDK3 interacts with a yet unknown type of cyclin. It can phosphorylate histone H1. Endogenous Cyclin C/Cdk3 complexes are critical to promote exit from quiescence in an Rb-dependent manner. Cyclin C/Cdk3 complexes is a key regulators of cell cycle reentry in human cells. (PMID: 15130482 )The antibody is specific to CDK3.

Publications

Species	Application	Title
human	WB	Cell Chem Biol; CDK2 heterobifunctional degraders co-degrade CDK2 and cyclin E resulting in efficacy in CCNE1-amplified and overexpressed cancers; Authors - Nicholas Kwiatkowski
human	IHC	Cancer Med; Identification of an EMT-related gene-based prognostic signature in osteosarcoma; Authors - Haoli Gong
mouse	WB	Protein J; Characterization of Protein Profiling and mRNA Expression of LLC Exosomes.; Authors - Wen Zhang
human	WB	Cell Death Discov; Up-regulation of SLC27A2 suppresses the proliferation and invasion of renal cancer by down-regulating CDK3-mediated EMT.; Authors - Ning Xu
human	WB	Cancer Med; The expression and role of SUZ12 in lung adenocarcinoma; Authors - Xingsheng Hu

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