Anticorps Polyclonal de lapin anti-CDK8

• Phare
• Validé par KD/KO

CDK8 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 22067-1-AP

Synonymes

CDK8, Cell division protein kinase 8, cyclin dependent kinase 8, K35, Mediator complex subunit CDK8, Protein kinase K35

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Galerie de données de validation

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  WB analysis using 22067-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22067-1-AP (CDK8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22067-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22067-1-AP (CDK8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22067-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22067-1-AP (CDK8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 22067-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 22067-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 22067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 22067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of NIH/3T3 using 22067-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 22067-1-AP (CDK8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of NIH/3T3 using 22067-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human CDK8 (22067-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules NIH/3T3, tissu embryonnaire de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3
• Résultats positifs en cytométrie: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

22067-1-AP cible CDK8 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDK8 Protéine recombinante Ag17148
• Nom complet: cyclin-dependent kinase 8
• Masse moléculaire calculée: 464 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC069634
• Symbole du gène: CDK8
• Identification du gène (NCBI): 1024
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; Loss of MED12 activates the TGFβ pathway to promote chemoresistance and replication fork stability in BRCA-deficient cells.; Authors - Lindsey M Jackson ; KD Validated
human	WB	PLoS Genet; Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.; Authors - Emily M Schleicher ; KD Validated
human	WB	Biochim Biophys Acta Gene Regul Mech; PP2A and its adapter protein IER5 induce the DNA-binding ability and target gene expression of E2F1 via dephosphorylation at serine 375; Authors - Hiroto Takeuchi
mouse	WB,IHC	Bull Exp Biol Med; Knockdown of Long Noncoding RNA CCAT2 Suppresses Malignant Phenotype in Human Laryngeal Squamous Cell Carcinoma; Authors - M Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SITING (Verified Customer) (04-02-2021)

can see band between 50-65kd

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: EMBRYO HEART

CDK8 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:22067-1-AP)