Anticorps Polyclonal de lapin anti-CDKAL1

CDKAL1 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 22988-1-AP

Synonymes

CDKAL1

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Galerie de données de validation

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  WB analysis of mouse lung using 22988-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22988-1-AP (CDKAL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 22988-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22988-1-AP (CDKAL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 22988-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 22988-1-AP (CDKAL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22988-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22988-1-AP (CDKAL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22988-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22988-1-AP (CDKAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus using 22988-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus slide using 22988-1-AP (CDKAL1 Antibody) at dilution of 1:50.

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  IHC staining of human oesophagus using 22988-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus slide using 22988-1-AP (CDKAL1 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 22988-1-AP

  Immunofluorescent analysis of HepG2 cells using 22988-1-AP (CDKAL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules HepG2, cellules HT-1080, cellules Jurkat
• Résultats positifs en IHC: tissu œsophagien humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

22988-1-AP cible CDKAL1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDKAL1 Protéine recombinante Ag19060
• Nom complet: CDK5 regulatory subunit associated protein 1-like 1
• Masse moléculaire calculée: 579 aa, 65 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC121020
• Symbole du gène: CDKAL1
• Identification du gène (NCBI): 54901
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cell cycle progression is controlled, in part, by a family of cyclin dependent kinases (Cdks) that work to phosphorylate key substrates involved in each phase of cell cycle progression. Cdks are the catalytic subunits of serine/threonine protein kinases, a large family of proteins that act as regulators of the eukaryotic cell cycle. CDKAL1 (Cdk5 regulatory subunit associated protein 1-like 1) is a 579 amino acid single-pass membrane protein that contains one TRAM domain and is similar to Cdk5 regulatory subunit associated proteins (CDK5RAPs). Expressed in pancreas, brain and skeletal muscle, CDKAL1 uses iron as a cofactor and is involved in glucose-stimulated INS secretion. Defects in the gene encoding CDKAL1 impair INS secretion and are associated with the development of type 2 diabetes. Multiple isoforms of CDKAL1 exist due to alternative splicing events. This antibody recognizes the 65kd isoform of CDKAL1.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); CDKAL1 Drives the Maintenance of Cancer Stem-Like Cells by Assembling the eIF4F Translation Initiation Complex; Authors - Rongsheng Huang