Anticorps Polyclonal de lapin anti-CDO1

CDO1 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 12589-1-AP

Synonymes

CDO, CDO I, CDO1, Cysteine dioxygenase type 1, cysteine dioxygenase, type I

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Galerie de données de validation

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  WB analysis of mouse liver using 12589-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12589-1-AP (CDO1 antibody) at dilution of 1:3000 incubated at 4 degree celsius over night.

• 

  WB analysis of rat liver using 12589-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12589-1-AP (CDO1 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse liver using 12589-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12589-1-AP (CDO1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 12589-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12589-1-AP (CDO1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 12589-1-AP

  IP Result of anti-CDO1 (IP:12589-1-AP, 3ug; Detection:12589-1-AP 1:600) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12589-1-AP (CDO1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12589-1-AP (CDO1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12589-1-AP (CDO1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12589-1-AP (CDO1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12589-1-AP (CDO1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12589-1-AP (CDO1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12589-1-AP (CDO1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 12589-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12589-1-AP (CDO1 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du pancréas humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 6 publications below

Informations sur le produit

12589-1-AP cible CDO1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDO1 Protéine recombinante Ag3294
• Nom complet: cysteine dioxygenase, type I
• Masse moléculaire calculée: 200 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC024241
• Symbole du gène: CDO1
• Identification du gène (NCBI): 1036
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDO1(cysteine dioxygenase type 1) is also named as CDO and belongs to the cysteine dioxygenase family. It is an enzyme that adds molecular oxygen to the sulfur of cysteine, converting the thiol to a sulfinic acid known as cysteinesulfinic acid (3-sulfinoalanine) and CDO1 is one of the most highly regulated metabolic enzymes responding to diet(PMID:19011731). The expression of CDO can significantly decrease the level of intracellular cysteine and this reduction is also associated with a decrease in total glutathione levels(PMID:17327371). Cysteine dioxygenase (CDO) plays a critical role in the regulation of cellular cysteine concentration andmultiple forms of CDO (23 kDa, 25 kDa, and 68 kDa) have been claimed based upon separation and detection using SDS-PAGE/western blotting(PMID: 14752623).

Publications

Species	Application	Title
human	WB,IHC	Cell Death Discov; Cysteine dioxygenase 1 attenuates the proliferation via inducing oxidative stress and integrated stress response in gastric cancer cells; Authors - Gang Ma
human	IHC	Int J Biol Sci; Ferroptosis-associated molecular classification characterized by distinct tumor microenvironment profiles in colorectal cancer.; Authors - Wenqin Luo
mouse	WB	J Nutr Biochem; PMID: 24917045; Authors - Kerstin E Geillinger
rat	WB	J Ethnopharmacol; Integration of metabolomics and transcriptomics to reveal anti-chronic myocardial ischemia mechanism of Gualou Xiebai decoction.; Authors - Fengyun Zhang
human	IHC	Ann Surg Oncol; Promoter DNA Hypermethylation of the Cysteine Dioxygenase 1 (CDO1) Gene in Intraductal Papillary Mucinous Neoplasm (IPMN).; Authors - Yoshiki Fujiyama
mouse	WB	Cell Mol Neurobiol; Taurine Alleviates Chronic Social Defeat Stress-Induced Depression by Protecting Cortical Neurons from Dendritic Spine Loss.; Authors - Yuanyuan Zhu