Anticorps Polyclonal de lapin anti-CDR2L

• Phare
• Validé par KD/KO

CDR2L Polyclonal Antibody for IF, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14563-1-AP

Synonymes

CDR2L, HUMPPA, Paraneoplastic 62 kDa antigen

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Galerie de données de validation

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  WB analysis of K-562 using 14563-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14563-1-AP (CDR2L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14563-1-AP

  WB result of CDR2L antibody (14563-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDR2L transfected SH-SY5Y cells.

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  IP experiment of SH-SY5Y using 14563-1-AP

  IP Result of anti-CDR2L (IP:14563-1-AP, 3ug; Detection:14563-1-AP 1:500) with SH-SY5Y cells lysate 2500ug.

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  IHC staining of human cerebellum using 14563-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14563-1-AP (CDR2L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cerebellum using 14563-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14563-1-AP (CDR2L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14563-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14563-1-AP (CDR2L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14563-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14563-1-AP (CDR2L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14563-1-AP

  Immunofluorescent analysis of HepG2 cells using 14563-1-AP (CDR2L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IP: cellules SH-SY5Y
• Résultats positifs en IHC: tissu de cervelet humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

14563-1-AP cible CDR2L dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDR2L Protéine recombinante Ag6127
• Nom complet: cerebellar degeneration-related protein 2-like
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC047534
• Symbole du gène: CDR2L
• Identification du gène (NCBI): 30850
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cerebellar degeneration-related protein 2-like(CDR2L), also named as HUMPPA or Paraneoplastic 62 kDa antigen, is a 465 amino acid protein, which belongs to the CDR2 family. CDR2L contains three potential coiled-coil regions. The functions of protein is so far not known. CDR2L is expressed in the brain and localizes in the cytoplasm. The predicted MW of this protein is 53 kDa.

Publications

Species	Application	Title
human	IP, WB	Acta Neuropathol; Paraneoplastic CDR2 and CDR2L antibodies affect Purkinje cell calcium homeostasis.; Authors - Manja Schubert
human	WB,IHC	Cancer Immunol Immunother; Cerebellar degeneration-related proteins 2 and 2-like are present in ovarian cancer in patients with and without Yo antibodies.; Authors - Margrethe Raspotnig
human	WB,IF,IP	Ann Clin Transl Neurol; Localization of CDR2L and CDR2 in paraneoplastic cerebellar degeneration.; Authors - Ida Herdlevaer
human,rat	WB,IHC	J Neuroimmunol; Expression of cerebellar degeneration-related proteins CDR2 and CDR2L in human and rat brain tissue.; Authors - Margrethe Raspotnig