Anticorps Monoclonal anti-CDR2L

• Phare
• Validé par KD/KO

CDR2L Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2E12D10

N° de cat : 66791-1-Ig

Synonymes

CDR2L, HUMPPA, Paraneoplastic 62 kDa antigen

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Galerie de données de validation

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  WB analysis of U-251 using 66791-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66791-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66791-1-Ig

  WB result of CDR2L antibody (66791-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDR2L transfected HEK-293 cells.

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  WB analysis of mouse brain using 66791-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 66791-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66791-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66791-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cerebellum using 66791-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66791-1-Ig (CDR2L antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human cerebellum using 66791-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66791-1-Ig (CDR2L antibody) at dilution of 1:400 (under 40x lens).

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  IF Staining of HEK-293 using 66791-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CDR2L antibody (66791-1-Ig, Clone: 2E12D10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U-251, cellules HEK-293, cellules K-562, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IHC: tissu de cervelet humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IP: See 1 publications below

Informations sur le produit

66791-1-Ig cible CDR2L dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDR2L Protéine recombinante Ag6139
• Nom complet: cerebellar degeneration-related protein 2-like
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC047534
• Symbole du gène: CDR2L
• Identification du gène (NCBI): 30850
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cerebellar degeneration-related protein 2-like(CDR2L), also named as HUMPPA or Paraneoplastic 62 kDa antigen, is a 465 amino acid protein, which belongs to the CDR2 family. CDR2L contains three potential coiled-coil regions. The functions of protein is so far not known. CDR2L is expressed in the brain and localizes in the cytoplasm.

Publications

Species	Application	Title
human	IP	Ann Clin Transl Neurol; Localization of CDR2L and CDR2 in paraneoplastic cerebellar degeneration.; Authors - Ida Herdlevaer

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