Anticorps Polyclonal de lapin anti-CEA

• Phare
• Validé par KD/KO

CEA Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, chIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10421-1-AP

Synonymes

Carcinoembryonic antigen, CD66e, CEA, CEACAM5, Meconium antigen 100

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Galerie de données de validation

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  WB analysis using 10421-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10421-1-AP (CEA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10421-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10421-1-AP (CEA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10421-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10421-1-AP (CEA antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10421-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10421-1-AP (CEA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10421-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10421-1-AP (CEA antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 10421-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10421-1-AP (CEA antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach cancer using 10421-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10421-1-AP (CEA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human appendicitis using 10421-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10421-1-AP (CEA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10421-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10421-1-AP (CEA antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of human colon cancer using 10421-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CEA antibody (10421-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human colon cancer using 10421-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CEA antibody (10421-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules MCF-7, salive humain, salive humaine
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu d'appendicite humain, tissu de cancer de l'estomac humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du côlon humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• ChIP: See 1 publications below

Informations sur le produit

10421-1-AP cible CEA dans les applications de WB, IHC, IF, chIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEA Protéine recombinante Ag0672
• Nom complet: carcinoembryonic antigen-related cell adhesion molecule 5
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 180-200 kDa, 77 kDa
• Numéro d’acquisition GenBank: BC034671
• Symbole du gène: CEACAM5
• Identification du gène (NCBI): 1048
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.

Publications

Species	Application	Title
human	WB	Transl Res; Molecular signature of interleukin-22 in colon carcinoma cells and organoid models.; Authors - Ina Rudloff
human	WB,IHC,chIP	Int J Biol Sci; FBW7 suppresses metastasis of colorectal cancer by inhibiting HIF1α/CEACAM5 functional axis.; Authors - Qingguo Li ; KD Validated
human	IHC	Gene Ther; Lentivirus-mediated RASSF1A expression suppresses aggressive phenotypes of gastric cancer cells in vitro and in vivo.; Authors - P-H Zhou
human	IHC	World Neurosurg; Discovery of Aquaporin-1 and Aquaporin-4 Expression in an Intramedullary Spinal Cord Ependymal Cyst: Case Report.; Authors - Matthew J Shepard
human	WB	PLoS One; Correlation of alpha-1 antitrypsin levels and exosome associated neutrophil elastase endothelial injury in subjects with SARS-CoV2 infection; Authors - Jorge Lascano
mouse	WB	Iran J Basic Med Sci; Preparation and application of patient-derived xenograft mice model of colorectal cancer; Authors - Yutao Zhang

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