Anticorps Polyclonal de lapin anti-CEP170

• Phare
• Validé par KD/KO

CEP170 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

Avis (2)

N° de cat : 27325-1-AP

Synonymes

centrosomal protein 170kDa, Centrosomal protein of 170 kDa, CEP170, FAM68A, KAB, KARP 1 binding protein, KARP1 binding protein, KIAA0470

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Galerie de données de validation

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  WB analysis of MCF-7 using 27325-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27325-1-AP (CEP170 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27325-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27325-1-AP (CEP170 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 27325-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 27325-1-AP (CEP170 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 6 publications below

Informations sur le produit

27325-1-AP cible CEP170 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEP170 Protéine recombinante Ag26311
• Nom complet: centrosomal protein 170kDa
• Masse moléculaire calculée: 1486 aa, 165 kDa
• Poids moléculaire observé: 170-190 kDa
• Numéro d’acquisition GenBank: BC140794
• Symbole du gène: CEP170
• Identification du gène (NCBI): 9859
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Science; A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.; Authors - Chun So
	WB	Nat Commun; CEP128 is involved in spermatogenesis in humans and mice.; Authors - Xueguang Zhang
human	IF	J Cell Biol; DNA damage promotes microtubule dynamics through a DNA-PK-AKT axis for enhanced repair.; Authors - Shuyun Ma
human	WB,IF	Elife; α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.; Authors - Dandan Ma ; KD Validated
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu
human	WB,IF	Reprod Biomed Online; A novel CCDC39 mutation causes multiple morphological abnormalities of the flagella in a primary ciliary dyskinesia patient; Authors - Daijuan Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ilham (Verified Customer) (08-06-2024)

CEP170 works very well I confirm that the observed molecular weight is higher than the calculated molecular weight, using at 1:1000 in 5% milk TBSt O/N (LI-COR).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Laura (Verified Customer) (02-27-2019)

Comparing with antibodies from other companies that we bought, this antibody is the best that we tried for IF and Western Blot !

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: if 1/200
• Cell Tissue Type: Rpe1

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