Anticorps Polyclonal de lapin anti-CEP290

• Phare
• Validé par KD/KO

CEP290 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (1)

N° de cat : 22490-1-AP

Synonymes

3H11Ag, BBS14, Cancer/testis antigen 87, centrosomal protein 290kDa, Centrosomal protein of 290 kDa, CEP290, CT87, JBTS5, JBTS6, KIAA0373, LCA10, MKS4, Nephrocystin 6, rd16, SLSN6, Tumor antigen se2 2

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Galerie de données de validation

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  WB analysis of HeLa using 22490-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22490-1-AP (CEP290 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22490-1-AP

  IP result of anti-CEP290(IP:22490-1-AP, 4ug; Detection:22490-1-AP 1:3000) with HeLa cells lysate 1320 ug.

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  IHC staining of human prostate cancer using 22490-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22490-1-AP (CEP290 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 22490-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22490-1-AP (CEP290 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 22490-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22490-1-AP (CEP290 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 8 publications below
• IP: See 1 publications below

Informations sur le produit

22490-1-AP cible CEP290 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEP290 Protéine recombinante Ag17457
• Nom complet: centrosomal protein 290kDa
• Masse moléculaire calculée: 2479 aa, 290 kDa
• Poids moléculaire observé: 290 kDa
• Numéro d’acquisition GenBank: BC008641
• Symbole du gène: CEP290
• Identification du gène (NCBI): 80184
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Studies in the Chlamydomonas model system has shown that the ciliary protein CEP290 is a critical component of these Y-link junctions. Additionally, numerous studies have demonstrated that CEP290's function is critical for IFT-in CEP290 knockdown experiments, many proteins that would normally localize to the cilium fail to do so, and cilium formation is disrupted or absent. Mutations in CEP290 are accountable for cases of nephronophthisis, Leber congenital amaurosis and Joubert syndrome. In IF analtsis of HeLa cells, the white arrows show centrosome and cilium staining. Two isoforms were produced by alternative splicing with predicted MW of 290 and 180 kDa according to UniProt. Catalog#22490-1-AP can recognise both.

Publications

Species	Application	Title
mouse	IF	J Cell Biol; The small GTPase RSG1 controls a final step in primary cilia initiation.; Authors - Stephanie O Agbu
mouse	WB	Cell Rep; A CEP290 C-Terminal Domain Complements the Mutant CEP290 of Rd16 Mice In Trans and Rescues Retinal Degeneration.; Authors - Suddhasil Mookherjee
human	IF	EMBO Rep; Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy.; Authors - Andrea Martello
human	WB,IHC,IP	Aging (Albany NY); Centrosomal protein 290 is a novel prognostic indicator that modulates liver cancer cell ferroptosis via the Nrf2 pathway.; Authors - Yiru Shan ; KD Validated
human	WB,IF	J Biol Chem; DAZ interacting protein 1 (Dzip1) phosphorylation by Polo-like kinase 1 (Plk1) regulates the centriolar satellites localization of the BBSome during the cell cycle.; Authors - Boyan Zhang
mouse	WB,IF	J Biol Chem; The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.; Authors - Poppy Datta ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jay (Verified Customer) (11-08-2019)

Human RPE1 cell line with Methanol fixation.Immunostaining result shows that CEP290 localizes to centrosome (Red; CEP290, Green; CEP164 for Centrosome marker, Blue;DAPI for Nucleus).The antibody seems to detect additional nucleoli signals.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human RPE1

CEP290 Antibody Immunofluorescence, validation (1:100 dilution) in Human RPE1 (Cat no:22490-1-AP)