Anticorps Polyclonal de lapin anti-CEP63

• Phare
• Validé par KD/KO

CEP63 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 16268-1-AP

Synonymes

centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63

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Galerie de données de validation

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  WB analysis of HEK-293 using 16268-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16268-1-AP (CEP63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 16268-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16268-1-AP (CEP63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16268-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16268-1-AP (CEP63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16268-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16268-1-AP (CEP63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16268-1-AP

  IP Result of anti-CEP63 (IP:16268-1-AP, 3ug; Detection:16268-1-AP 1:500) with HEK-293 cells lysate 1700ug.

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  IF Staining of MDCK using 16268-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CEP63 antibody (16268-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu de thymus de souris, tissu hépatique de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IF: cellules MDCK,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 9 publications below
• IP: See 3 publications below

Informations sur le produit

16268-1-AP cible CEP63 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEP63 Protéine recombinante Ag9335
• Nom complet: centrosomal protein 63kDa
• Masse moléculaire calculée: 703 aa, 81 kDa
• Poids moléculaire observé: 57-75 kDa
• Numéro d’acquisition GenBank: BC014050
• Symbole du gène: CEP63
• Identification du gène (NCBI): 80254
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CEP63 is a 63 kDa centrosomal protein. It is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response. This antibody can recognize all the four isoforms of CEP63 (56 kDa, 58 kDa, 63 kDa and 81 kDa).

Publications

Species	Application	Title
mouse		Nat Cell Biol; Massive centriole production can occur in the absence of deuterosomes in multiciliated cells.; Authors - Olivier Mercey
mouse	WB,IF	Neuron; Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.; Authors - Divya Jayaraman
human	WB, IF, IP	Dev Cell; A dual role for UVRAG in maintaining chromosomal stability independent of autophagy.; Authors - Zhen Zhao ; KO Validated
mouse	IF	Nat Commun; Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.; Authors - Munemasa Mori
human	IF	Nat Commun; Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites.; Authors - Søs Grønbæk Holdgaard
mouse	IF	Nat Commun; Autophagy controls centrosome number by degrading Cep63.; Authors - Yuichiro Watanabe